Muhammad Tariq Pervaz
Virtual University 54 Lawrence, Pakistan
Title: Hepatitis B Virus Genotype D Sub Genotype D1 Circulating in Pakistan, China and India: An In-Silico Analysis.
Biography
Biography: Muhammad Tariq Pervaz
Abstract
HBV genotype D sub genotype D1 predominates in Pakistan and India. It is more than 80% among all genotypes of HBV in these two regions. However, it is less dominant in China where genotype B and C predominates. In this study, we present a computational analysis of HBV genotype D sub genotype D1 in Pakistan, India and China. For this purpose, 25 complete genome sequences of HBV genotype D sub genotype D1 were collected. Four complete genome sequences were obtained from Pakistan 8 from China and 13 from India. Sequence alignment showed less than 4% divergence in reported sequences from Pakistan, India and China. C and X genes showed divergence of less than 3%. While comparison over the S gene showed similarity ratio of 97–98% of genotype D sub genotype D1. Phylogenetic analysis highlighted that Pakistan HBV complete genome isolate have the closest evolutionary relationship with the HBV complete genome isolate prevailing in its neighbor countries China and India. Gene structure analysis showed that exons of ‘P’ gene was the longest; about 75% of the gene size while gene ‘S’ had 2nd longest coding regions with two exons and one intron with size ratio of 40% and 60% respectively.